U of u clinics

BIOMEDIA: Publishing services - Clinical Biochemistry, n. cognitive Indagine on the spread of the analyses of molecular Biology in the laboratories clinicians of the region Marine Puglia Micelli *, Mario Correale, Antonio Iacobacci, Sergio Giannattasio * Laboratory of Coagulazione-Azienda Policlinico Hospital worker, 70124 Bariums. Determination of the lipoamide dehydrogenates in the serum: proposal of a new analytical-instrumental approach and comparison with other pointers of mitocondriale lesion Serene Cristina *, Roberto Bonora, Mauro Panteghini * Laboratory Clinical Chemical Analyses 1, Company Hospital worker ' Spedali Civili', Brescia. The lipoamide dehydrogenase (LAD) is to mitochondrial flavoprotein which catalyzes the reduction of lipoamide by NADH to yield dihydrolipoamide and NAD+. The enzyme is to component of the multienzyme pyruvate dehydrogenase complex and 2-oxoglutarate dehydrogenase complex. LAD can be measured in human serum and used potentially as marker of tissue damage. In order to investigated the clinical utility of LAD determination, we adapted to modification of the manual method of Pelley ET to. (Clin Chem 1976.22:275) for use on the Roche Cobas Bio analyzer. The assay measures the lipoamide-dependent reduction of NAD+ at 340 nm. The automated assay requires 15 mililiter of specimen and 140 mililiter of reagents containing NAD+ and reduced lipoamide in tricine buffer, 50 mmol/L. Following 10 min incubation, seven absorbance readings to were taken at 10 s intervals (37°.C). Under the conditions described, the products of reaction increased linearly with serum LAD activities up to 300 U/L. The average percentage recovery of various quantities of commercial LAD (Sigma) was 98. Analysis of total imprecision, using three NCCLS-EP5 protocol on human evening with 15. Triglycerides, hemoglobin, and bilirubin I give not interfere up to values of 500 mg/dL, 0. Those Results of LAD obtained with our method (y) well correlated with of manual method of Pelley (x) (r=0.9947) yielding the following regression equation: y = 1. The upper reference limit, determined in to group of 33 ostensibly healthy subjects, was 19 U/L (97, given From presented, we concludes that our automated method may now permit studies on the clinical utility of serum LAD. Marking biochemists of the bony metabolism in adult patients with morbo celiaco. Laboratory of Clinical Chemistry "Baldi and Riberi", Company Saint Hospital worker Giovanni Batiste, Turin. Criteria According to WHO, 34% of patients had to normal BMD, 40% had osteopenia and 26% osteoporosis. Osteopenics had higher bone specific alkaline phosphatase (BAP) and osteocalcin values than subjects with to normal BMD. You go to the Index of Clinical Biochemistry For who wishes to publish on BC, they are available For who wishes to underwrite the subscription to BC?isponibile For observations and the comments on the WWW services to contact for technical problems sent a mail to. 1997, BIOMEDIA srl, all the straight ones reserve The you material on this situated may not be reproduced or otherwise used without the prior permission of BIOMEDIA.